Optimization of the Fermentation Conditions of Metarhizium robertsii and Its Biological Control of Wolfberry Root Rot Disease

Fusarium solani is the main pathogenic fungus causing root rot of wolfberry (Lycium barbarum). The endophytic Metarhizium robertsii has been widely used for biocontrol plant fungi, but effects this on and its antifungal mechanism against F. have not reported. In study, antagonism M. was verified. Further, we optimized fermentation conditions broth based inhibition rate solani. addition, partial were investigated. results showed that exhibited good Glucose beef extracts optimal carbon nitrogen sources robertsii. Under 29 °C, 190 rpm, pH 7.0, had best effect Furthermore, treatment decreased activities superoxide dismutase, catalase, peroxidase solani; promoted accumulation malondialdehyde; accelerated leakage soluble protein decrease in sugar. inoculation with significantly reduced decay incidence disease index caused by These indicate could be as a biological control agent management.